Modified
Dec 2012
RW

Advances in Free Flow Electrophorsis since 35 years

      Free Flow Electrophoresis Systems for high resolution separations

March 27, 2013, application development:

-  Top story part I, separation of Thylakoid membranes and the importance of sample preparation

- comparison of DDM and Digitonin based sample preparation, followed by interval Zone Electrophoresis
- visualization by BN-PAGE and SDS-PAGE

click here to learn more about it

December 5, 2012, recent developments:

-  add-on robotic system launched:

Specifications:

• automated operation of single experiments for interval mode and continuous mode
   
• automated handling of up to three MTPs
   
• software control of the FFE instrument and robotic system
   
• enables reproducible interval runs
   
• facilitates experiments and handling
   
• compatible with new FFE systems and with instruments of the Prometheus and BD line

novel applications available:

• separation of isoforms in ultra-flat gradients using either commercial Ampholytes or ProLyte reagents
  
  we developed new separation buffers and protocols for ultra-flat pH gradients to enable high resolution protein
  isoform separation
   
• Poster available for download, shown at CE Pharm congress, October 2012

February 21s, 2012t, recent developments:  Automated FFE platform launched:

• Features FFE:

• Increased fieldstregth of >800V/cm allow separation speeds of less than 1 Minute
• New gap size, down to 0.175 mm offers new dimensions of resolution
• New wall material allows to keep the separation chamber closed for five days  of operation

• Features robot

• automated Microplate handling for up to 64 MTPs
• automated sample handling of up to 22 samples
• automated separation buffer handling for up to 4 different protocols
• automated washing and cleaning steps
• software controlled, fully automated operation
• documentation and control of series of experiments
   

 July 2011, recent developments:

  Novel applications to increase  throughput and resolution of FFE separations are now available:

• Increased fieldstregth of >800V/cm allow separation speeds of less than 1 Minute
   
• New gap size, down to 0.175 mm offers new dimensions of resolution
   
• New wall material allows to keep the separation chamber closed for five days  of operation
   
• Electrophoretic sieving in polymer solution allows native purification of protein complexes and separation by size
   
• Ultra flat pH gradient IEF separation for high resulotion native isoform purification
   
  Results are published in the ‘Applications’ section.
  
  Existing instruments can be upgraded to get the benefits of the recent techological developments
  
   

  
   From March 2011 FFE Service GmbH will provid technical and applicational support  for  BD FFE systems
 

Free Flow Electrophoresis is a versatile technology to support key  applications for protein analysis:

• High resolution separations for protein isoforms
• Reduction of the complexity of the proteome before further proteomic analysis
• Access to low-abundant proteins
• Separation of cells, cellular organelles and sub-cellular fragments
• Continuous sample application to process large sample volumes
• High resolution matrix-free fractionation in up to 96 fractions
• Preparative native electrophoresis to bring a new dimension to protein interaction studies

The FFE system provides  researchers with a tool to reduce the complexity of samples and enrich low abundant proteins with highest reproducibility. Furthermore, FFE provides  a fluid-phase fractionation of cell organelles, proteins and other charged or chargeable entities on a fast, preparative and continuous basis.