IZE Ribosome Separation

Ribosomes

The analyte was a cell extract from Bacillus subtilis in the log phase.

The ribosomes were separated by interval zone electrophoresis

Four to five (results in in examination) different subspecies of ribosomes were detected.

The graph on the top shows the absorption at 260nm and 280nm.

The gel is SDS-PAGE of the fractions 13-72

M=protein marker

S=crude extract

FFE Settings:

IZE Mode
Settings: 1200 V
Transit time: 2.5 min