Free Flow Electrophoresis

The FFE system provides a fluid-phase fractionation of charged bioparticles, biopolymers or other charged or chargeable entities on a fast, preparative and continuous basis that guarantees best analyte recovery. New separation media were recently shown to support high-resolution separation and enrichment:

1. CHIEF-Separation protocols for amphoteric analytes:
   • FF-IEF with commercial ampholytes for separation of protein isoforms
2. ZE/IEF-Separation protocols for amphoteric and non amphoteric analytes:
   • FF-ZE to FF-IEF continuous switch within 1 experiment
   • 10 µg up to 400 mg protein/h separation of bioparticles, protein complexes, membrane proteins and of protein isoforms like antibodies
   • reduction of proteome complexity and the identification of low-abundant proteins
3. FF-IZE/IITP Separation Protocols for non amphoteric analytes:
   • non-amphoteric separation of analytes like Nano particles, liposomes, or polysulfonates, like Dendromeres
4. FF-ZE and FF-FSE Separation Protocols, using volatile media:
   • Enrichment of drug metabolites from water with a capacity of up to 100 ml/h
5. Various Separation Protocols for Exosomes from human plasma and cell culture media:
   • Heterogeneity of exosomes demonstrated with > 7 subfractions
   • Purity of exosome shown by ultrafiltration of FFE subfractions

FFE Service GmbH offers Free Flow Electrophoretic separations as service.

Feel free to contact us about instruments, separation services and or demonstations.